Low-level laser therapy for zymosan-induced arthritis in rats: Importance of illumination time
Importancia del tiempo de iluminacion en artritis inducida con Zymosan en ratas
Ana P. Castano, MD 1 2, Tianhong Dai, PhD 1 2, Ilya Yaroslavsky, PhD 3, Richard Cohen, MD 3, William A. Apruzzese, PhD 3, Michael H. Smotrich, PhD 3, Michael R. Hamblin, PhD 1 2 4 *
1Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114
2Department of Dermatology, Harvard Medical School, Boston, Massachusetts 02115
3Palomar Medical Technologies Inc., Burlington, Massachusetts 01803
4Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139
Background
It has been proposed for many years that low-level laser (or light) therapy (LLLT) can ameliorate the pain, swelling, and inflammation associated with various forms of arthritis. Light is thought to be absorbed by mitochondrial chromophores leading to an increase in adenosine triphosphate (ATP), reactive oxygen species and/or cyclic AMP production and consequent gene transcription via activation of transcription factors. However, despite many reports about the positive effects of LLLT in arthritis and in medicine in general, its use remains controversial. For all indications (including arthritis) the optimum optical parameters have been difficult to establish and so far are unknown.
Methods
We tested LLLT on rats that had zymosan injected into their knee joints to induce inflammatory arthritis. We compared illumination regimens consisting of a high and low fluence (3 and 30 J/cm2), delivered at high and low irradiance (5 and 50 mW/cm2) using 810-nm laser light daily for 5 days, with the positive control of conventional corticosteroid (dexamethasone) therapy.
Results
Illumination with 810-nm laser was highly effective (almost as good as dexamethasone) at reducing swelling and a longer illumination time (10 or 100 minutes compared to 1 minute) was more important in determining effectiveness than either the total fluence delivered or the irradiance. LLLT induced reduction of joint swelling correlated with reduction in the inflammatory marker serum prostaglandin E2 (PGE2).
Conclusion
LLLT with 810-nm laser is highly effective in treating inflammatory arthritis in this model. Longer illumination times were more effective than short times regardless of total fluence or irradiance. These data will be of value in designing clinical trials of LLLT for various arthritides. Lasers Surg. Med. 39:543-550, 2007. © 2007 Wiley-Liss, Inc.
Funded by:
Palomar Medical Technologies Inc.
U.S. National Institutes of Health; Grant Number: R01-CA/AI838801, R01-AI050875
Department of Defense CDMRP Breast Cancer Research; Grant Number: W81XWH-04-1-0676
biostimulation • low-level light therapy • zymosan-induced arthritis • photobiomodulation • cold laser • prostaglandin E2
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